CC-Sim: Appendix

Sources

CHO Cell stages, size increase, composition Pan X, Dalm C, Wijffels RH, Martens DE. Metabolic characterization of a CHO cell size increase phase in fed-batch cultures. Appl Microbiol Biotechnol. 2017;101(22):8101-8113. doi:10.1007/s00253-017-8531-y

Shear equations: R. Bowen, Unraveling the mysteries of shear-sensitive mixing systems, Chem. Eng. 9 (June) (1986) 55–63.

kLa equations: Liu, K.; Phillips, J.R.; Sun, X.; Mohammad, S.; Huhnke, R.L.; Atiyeh, H.K. Investigation and Modeling of Gas-Liquid Mass Transfer in a Sparged and Non-Sparged Continuous Stirred Tank Reactor with Potential Application in Syngas Fermentation. Fermentation 2019, 5, 75.

CO2 Equilibria: https://folk.ntnu.no/skoge/prost/proceedings/dycops2007-and-cab2007/CAB/Monday/Estimation/M32_89_Final_ManuscriptStampedno.pdf

OUR and CO2UR https://aiche.onlinelibrary.wiley.com/doi/abs/10.1002/btpr.646

Chemically Defined Media: Pan X, Streefland M, Dalm C, Wijffels RH, Martens DE. Selection of chemically defined media for CHO cell fed-batch culture processes. Cytotechnology. 2017;69(1):39-56. doi:10.1007/s10616-016-0036-5

Blunt W, Gaugler M, Collet C, et al. Rheological Behavior of High Cell Density Pseudomonas putida LS46 Cultures during Production of Medium Chain Length Polyhydroxyalkanoate (PHA) Polymers. Bioengineering (Basel). 2019;6(4):93. Published 2019 Oct 9. doi:10.3390/bioengineering6040093

S. Schaepe, A. Kuprijanov, C. Sieblist, M. Jenzsch, R. Simutis, A. Lübbert, kLa of stirred tank bioreactors revisited, Journal of Biotechnology, Volume 168, Issue 4, 2013, Pages 576-583, ISSN 0168-1656, https://doi.org/10.1016/j.jbiotec.2013.08.032.

Sieblist C, Hägeholz O, Aehle M, Jenzsch M, Pohlscheidt M, Lübbert A. Insights into large-scale cell-culture reactors: II. Gas-phase mixing and CO₂ stripping. Biotechnol J. 2011;6(12):1547-1556. doi:10.1002/biot.201100153

Newton, Joseph & Vlahopoulou, Joanna & Zhou, Yuhong. (2017). Investigating and Modelling the Effects of Cell Lysis on the Rheological Properties of Fermentation Broths. Biochemical Engineering Journal. 121. 10.1016/j.bej.2017.01.009.

Popovic, M., Papalexiou, A., & Reuss, M. (1983). Gas residence time distribution in stirred tank bioreactors. Chemical Engineering Science, 38(12), 2015–2025. doi:10.1016/0009-2509(83)80105-5 

Qi, H. Membrane and Micro-sparging Aerations in Long-term High-density Perfusion Cultures of Animal Cells.  PhD Thesis. 2001; https://core.ac.uk/download/pdf/10192811.pdf